A 3D human neural cell culture system for modeling Alzheimer's disease
نویسندگان
چکیده
منابع مشابه
A novel 3D human glioblastoma cell culture system for modeling drug and radiation responses
Background Glioblastoma (GBM) is the most common primary brain tumor, with dismal prognosis. The failure of drug-radiation combinations with promising preclinical data to translate into effective clinical treatments may relate to the use of simplified 2-dimensional in vitro GBM cultures. Methods We developed a customized 3D GBM culture system based on a polystyrene scaffold (Alvetex) that rec...
متن کاملModeling lymphangiogenesis in a 3D-culture system
Unraveling the molecular mechanisms of lymphangiogenesis is hampered by the lack of appropriate in vitro models of three-dimensional (3D) lymph vessel growth which can be used to exploit the potential of available transgenic mice. We developed a potent reproducible and quantifiable 3D-culture system of lymphatic endothelial cells, the lymphatic ring assay, bridging the gap between 2D-in vitro a...
متن کامل3D Porous Calcium-Alginate Scaffolds Cell Culture System Improved Human Osteoblast Cell Clusters for Cell Therapy
Age-related orthopedic disorders and bone defects have become a critical public health issue, and cell-based therapy is potentially a novel solution for issues surrounding bone tissue engineering and regenerative medicine. Long-term cultures of primary bone cells exhibit phenotypic and functional degeneration; therefore, culturing cells or tissues suitable for clinical use remain a challenge. A...
متن کاملTowards human central nervous system in vitro models for preclinical research: strategies for 3D neural cell culture
Background The development of new drugs for human Central Nervous System (CNS) diseases has traditionally relied on 2D in vitro cell models and genetically engineered animal models. However, those models often diverge considerably from that of human phenotype (anatomical, developmental and biochemical differences) [1] contributing to a high attrition rate only 8% of CNS drugs entering clinical ...
متن کاملA 3D Sphere Culture System Containing Functional Polymers for Large-Scale Human Pluripotent Stem Cell Production
Utilizing human pluripotent stem cells (hPSCs) in cell-based therapy and drug discovery requires large-scale cell production. However, scaling up conventional adherent cultures presents challenges of maintaining a uniform high quality at low cost. In this regard, suspension cultures are a viable alternative, because they are scalable and do not require adhesion surfaces. 3D culture systems such...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Nature Protocols
سال: 2015
ISSN: 1754-2189,1750-2799
DOI: 10.1038/nprot.2015.065